| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Linder, Hayley E (15)
|
CTUN-Z |
25.50 |
26.43 |
0.93 |
| 2 |
Reinoso, Emily A (16)
|
CTUN-Z |
26.76 |
27.90 |
1.14 |
| 3 |
Kozma, Blanka (16)
|
MRYMID |
26.63 |
28.04 |
1.41 |
| 4 |
Wang, Chloe (17)
|
MRYMID |
27.00 |
28.05 |
1.05 |
| 5 |
Brew, Margaret C (14)
|
MRYMID |
27.46 |
28.57 |
1.11 |
| 6 |
Mendribil, Mia P (14)
|
CTUN-Z |
31.15 |
29.26 |
-1.89 |
| 7 |
Laurenceau, Alyssa M (13)
|
MRYMID |
30.50 |
29.73 |
-0.77 |
| 8 |
Popielarz, Victoria A (13)
|
CTZEUS |
29.27 |
30.15 |
0.88 |
| 9 |
Jung, Grace (16)
|
MRYMID |
30.30 |
30.54 |
0.24 |
| 10 |
Petropoulos, Caroline A (13)
|
CTZEUS |
30.18 |
31.03 |
0.85 |
| 11 |
Bayer, Cally (15)
|
MRYMID |
29.94 |
31.09 |
1.15 |
| 12 |
Becerra, NancyMaria (17)
|
MRYMID |
30.30 |
31.14 |
0.84 |
| 13 |
Belmar, Ayanna F (14)
|
MRYMID |
32.51 |
31.18 |
-1.33 |
| 14 |
Valerio, Sofia A (14)
|
MRYMID |
30.65 |
31.62 |
0.97 |
| 15 |
Kass, Gabrielle (16)
|
MRYMID |
31.74 |
32.61 |
0.87 |
| 16 |
Daniel, Alexis (14)
|
MRYMID |
32.42 |
33.02 |
0.60 |
| 17 |
Cahaly, Emma E (14)
|
MRYMID |
31.30 |
33.07 |
1.77 |
| 18 |
Rodriguez, Anna V (13)
|
MRYMID |
|
33.28 |
- |
| 19 |
Christinat, Elizabeth J (13)
|
CTZEUS |
37.10 |
35.58 |
-1.52 |
| 20 |
Tatar, Sophie E (13)
|
MRCOND |
35.30 |
35.99 |
0.69 |
| 21 |
Lagomarsino, Felicity J (14)
|
CTZEUS |
35.74 |
36.61 |
0.87 |
|
Khitrov, Samantha (13)
|
MRRA |
36.44 |
DQ |
- |